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. 2021 Mar 30;11:7197. doi: 10.1038/s41598-021-85477-y

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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In the absence of Myo1g, activated B lymphocytes have altered motility in a confined 3D microchannel. (a) A general model of the microfluidic chamber. This device has 96 channels with gap widths of 5, 10, and 15 μm. Each channel is 150 μm long. Briefly, LPS + IL-4 activated WT or Myo1g^−/− B lymphocytes are placed by gravity inside the chamber. Then, CXCL13 is applied on the opposite side. After that, migration is recorded by time-lapse microscopy. (b) Time of contact (s) of activated WT or Myo1g^−/− B lymphocytes with the 10 μm microchannel wall. (c) speed (μm/s) of activated B lymphocytes moving through a 10 μm microchannel calculated while the cells were moving within the microchannel. (d) Representative still images showing displacement of the cells over time; (red arrows). Data are presented as mean ± s.d. ****P < 0.0001.