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. 2021 Mar 30;11:7197. doi: 10.1038/s41598-021-85477-y

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Myo1g participates in the ability of B lymphocytes to generate membrane protrusions. (a) Representative images (63 × objective) of non-activated or LPS + IL-4 activated WT and Myo1g^−/− B lymphocytes incubated two hours on Fibronectin-covered coverslips. Cells were stained with Tritc-Phalloidin, and membrane protrusions are indicated with white arrows. Scale bars 5 μm. n = 3. (b) The number of membrane protrusions and (c) their lengths were measured on at least 50 activated B lymphocytes of WT and Myo1g^−/− mice in three independent experiments. They were analyzed using ImageJ software (National Institutes of Health, Bethesda, MD). Data correspond to three independent experiments. Data are presented as mean ± s.d. ****P < 0.0001.