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. 2021 Mar 30;12:1957. doi: 10.1038/s41467-021-22251-8

Fig. 3. Astigmatic tilted-CTF determination from low-dose tomographic tilt-series.

Fig. 3

a 2D power spectra from projections at −51°, 0°, and 51° from a tilt-series downloaded from EMPIAR-10453 (top) and corresponding fitted 1D models (bottom) showing radially averaged CTF profile (red), estimated defocus (green dashed line), and CTFfit scores (blue). b Astigmatic CTF estimation in the positive branch of a tilt-series from the dNTPase sample. Plots show variations in the astigmatism angle (green) and defocus differences (blue) across the tilt range (top). Corresponding 2D power spectra obtained from tilt angles 0, 21 and 36° showing changes in astigmatism (bottom). To facilitate the visualization, we choose to show a tilt-series with unusually high astigmatism variation. In general, no systematic variations in astigmatism were observed for data collected using BISECT or downloaded from the EMPIAR database.