Fig. 4. Self-tuning exposure weighting based on assigned particle scores.

a Average similarity scores calculated over individual particle projections and plotted as a function of the tilt-angle revealing the expected characteristic of the dose-symmetric scheme showing higher quality data obtained at the lower tilts. b 2D plot showing the cumulative contribution of individual tilts to the 3D reconstruction as a function of spatial frequency. Each colored band represents one tilt. The high frequency information is contributed mostly by lower tilts which are also exposed first.