Fig. 5. Near-atomic resolution structure of 300 kDa complex using BISECT/CSPT.

a Representative 50 nm-thick slice through tomographic reconstruction showing individual dNTPase particles (a total of 64 tomograms were used). Scale bar 100 nm. b Overview of dNTPase map obtained from ~30k particles extracted from 64 low-dose tomographic tilt-series. c Fourier Shell Correlation plots between half-maps showing an estimated resolution of 3.6 Å according to the 0.143-cutoff criteria. Curves corrected for masking effects (green dashed line), unmasked (magenta), masked (blue), and phase randomized (red) are shown. d Regions of the density map and fitted atomic coordinates showing the visualization of side chains. 3D refinement and reconstruction were repeated independently three times yielding structures with similar resolution.