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. 2021 Mar 30;12:1957. doi: 10.1038/s41467-021-22251-8

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a Representative 2D micrograph selected out of a total of 64 images (left, scale bar 100 nm), SPA map colored according to local resolution (middle) and corresponding density for an alpha helix (right). b Representative slice through tomographic reconstruction selected out of a total of 64 tomograms (left, scale bar 100 nm), ±36° BISECT/CSPT map colored according to local resolution (middle), and corresponding density for an alpha helix (right). 3D refinement and reconstruction were repeated independently three times yielding structures with similar resolution.