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. 2021 Mar 30;11:7199. doi: 10.1038/s41598-021-86658-5

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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PDPK1 KD does not effect cancer cell growth. (A) Western blot analysis of PDPK1 after 96 h KD of ERp57. (B) Western blot analysis of inducible PDPK1 KD in HCT116 cells. (C) MTT viability assay. Cells were incubated 96 h after PDPK1 KD before analysis. (D) Apoptosis was analysed by Caspase-3 activity assay 96 h after PDPK1 KD. (E) Colony formation assay after PDPK1 KD. Irradiation with 0, 1 and 3 Gy took place 24 h after seeding. Formed colonies were counted 10 d after seeding. Survival fraction is presented in bar graphs and representative images of colonies formed in 6-Well plates are shown.