Skip to main content
. 2021 Mar 30;11:7199. doi: 10.1038/s41598-021-86658-5

Figure 4.

Figure 4

Combination of ERp57 KD and chemical inhibition of PDPK1 does not effect cancer cell growth. (A) Colony formation assay after ERp57 KD in combination with PDPK1 inhibitor GSK 2334470 (3 µM). KD induction and inhibitor treatment was carried out right after seeding. The cells were irradiated 24 h after seeding. Formed colonies were counted 10 d after seeding. Survival fraction is presented in bar graphs and representative images of colonies formed in 6-Well plates are shown. (B) Western blot analysis of PDPK1 target p70S6K after 96 h of ERp57 KD and chemical inhibition of PDPK1 (3 µM GSK 2334470) or PI3K (50 µM LY294002) in normoxia. PCNA was used as a loading control. (C) MTT viability assay after 96 h of PDPK1 KD in combination with PDPK1 inhibition. (D) Western blot analysis after 96 h of PDPK1 KD in combination with chemical PDPK1 inhibition (3 µM GSK 2334470). For additional analysis of pPLK1, cells were treated with 0.1 µg/ml nocodazole 16 h before lysis to achieve G2/M arrest. DMSO was used as a vehicle control.