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. 2021 Mar 30;11:7199. doi: 10.1038/s41598-021-86658-5

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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KD of ERp57, but not PDPK1, impairs multiple proliferation factors independently of cellular oxygen levels. (A)–(C) Comparison of different proliferation factors in ERp57 KD and PDPK1 KD cells 96 h after induction of KD. (B) Cells were treated with 1 mM insulin 3 h before lysis. (C) To analyze pPLK1 in the G2/M phase, cells were treated with 0.1 µg/ml nocodazole 16 h before lysis. DMSO was used as a vehicle control.