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. 2021 Mar 30;12:1965. doi: 10.1038/s41467-021-22284-z

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Selected merged cell images for five histone PTMs. The red, green, and blue fluorescence spots each indicated the combination sites of 5hmU/PTM, 5fU/PTM, and 5hmC/PTM. The scale bar is 5 μm. b The spot percentages of three combination sites of single cells in five samples (cell numbers = 52, 34, 32, 43, and 38, respectively). c Analysis of the overlap or cluster of different spots. For the experiments of cell imaging in all figures, five times were repeated independently with similar results.