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. 2021 Mar 30;12:1971. doi: 10.1038/s41467-021-21984-w

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 6 — a, b RAW cells were treated as in Fig. 1 with mitochondria (Mito, 12 µg) (a) or EVs (from 10 × 10⁶ cells) (b) isolated from WT or OPA1 KO MEFs and IP10 release in culture media measured by ELISA. Individual points represent actual concentrations (or normalised to WT for (b), right panel) in independent experiments (Mito, n = 6; EVs, n = 4). Bars show the average ± SD. One-way ANOVA, two-sided t-test for (b), right panel. c OPA1 KO EVs show reduced expression of IFN-dependent genes. RAW cells were treated as in (a–b) and mRNA levels of the IFN-dependent genes Rsad2 (left) and mIfit1 (right) measured by qPCR. Individual points represent independent experiments (Rsad2, n = 6; mIfit1, n = 4). Bars show the average ± SD. One-way ANOVA. d, e RAW cells were treated as in (a, b) and IL6 was measured in the culture media by ELISA. Individual points represent independent experiments (Mito, n = 4, EVs, n = 5). Bars show the average ± SD. One-way ANOVA.