Figure 5.

DENV rEIII-induced mitochondria ROS production is ameliorated by Nlrp3 inflammasome and NETosis inhibitor. The rEIII (0.6 μM)-induced (1 h) elevations of neutrophil mitochondria mass (A) membrane potential (C) and superoxide (E) levels in a dose dependent manner. The induction of these mitochondria metabolic burdens in the neutrophils could be suppressed by treatments of Nlrp3 and caspase 1 inhibitors Z-WHED-FMK (10 μM) and OLT1177 (10 μM), NETosis inhibitor GSK484 (10 μM), and the cell-binding competitive inhibitor chondroitin sulfate B (CSB, 10 μg/mL), respectively (B,D,F). n = 6, *P < 0.05, **P < 0.01, vs. respective untreated vehicle groups (A,C,E); *P < 0.05, **P < 0.01, vs. respective vehicle groups (B,D,F).