Figure 6.

Nlrp3 inhibitor OLT1177 protects mice from DENV- and rEIII-induced NETosis and IL-1β, TNF-α production in mice. After challenged with vehicle (saline), BSA (a control protein) 2 mg/kg), DENV (1.2 × 10⁷ PFU /kg; DHF viral load) and rEIII (2 mg/kg; a dosage equivalent to DENV 1.2 × 10⁷ PFU/kg), experimental mice were immediately rescued with or without Nlrp3 inhibitor OLT1177 treatments (50 mg/kg). The ELISA analyzed plasma levels of circulating soluble citrullinated histone H3 (CitH3; a NETosis marker) (A), circulating IL-1β levels (B), and TNF-α levels (C) after 1 d treatments were showed. n = 6, ^##P < 0.01, vs. respective untreated groups; *P < 0.05, **P < 0.01, vs. respective vehicle groups.