Figure 7.

(A) shows representative flow cytometric data (above) and proportion (below) of FoXP3⁺ cells in CD4⁺ gate in T cells+ DCs, T cells+DCs+RvE1, T cells+ DCs+ PAM₃CSK₄ and T cells+ DCs+ PAM₃CSK₄+ RvE1. Naive CD4⁺ T cells were cocultured with dendritic cells and stimulated by PAM₃CSK_4, RvE1 treatment was applied baseline and on day 3 to the assigned groups. Cells were incubated at 37°C for five days, and on day 5. PMA/ionomycin and monensin were applied to the cells for 6 hours before performing FACS analysis. (B) CD11c+ dendritic cells (2x10^5 cells/500 𝓂l) were treated with 10 nM RvE1 for 24 hours and then induced with 100 ng PAM₃CSK₄ for 48 hours. One more dose of RvE1 was applied on day 2. (B) shows secreted cytokine levels by dendritic cells; Supernatants were collected on day 3; IL-6 and TGF_β were analyzed by AYOXXA and IL-2 by LUMINEX. Results shown are means ± sem for at least four independent experiments *p < 0.05, **p < 0.01.