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. 2021 Mar 30;11:7200. doi: 10.1038/s41598-021-86675-4

Figure1.

Figure1

Co-culture with embryonic intestinal stroma promotes differentiation of exogenous monocytes into CD11bhighCD11chigh CD103low MPs. (A) Mouse GFP + bone marrow cells were co-cultured ex vivo with wild type adult or embryonic intestinal stroma for 2 days. The differentiation state of live GFP + cells was analyzed by flow cytometry. T cells; CD3+, B cells; B220+, myelomonocytic cells; CD3-B220-CD11b+ n = 4 biological replicates. One way ANOVA with Tukey’s multiple test. *p < 0.05. (B) Representative bright field and fluorescent images of GFP labelled monocyte derived cells co-cultured with wild type intestinal adult or embryonic stromal cells for 2 days. Scale bar = 100 µm. (C) DAPI- GFP+ live monocyte derived cells from ex vivo coculture of stromal cells and monocytes shown in (B), were analyzed for CD11b and CD11c expression by flow cytometry. (D) Quantification of (C) showing the percentage of CD11bhighCD11chigh cells. n = 3 biological replicates. t-test. *p < 0.05. (E) Histogram showing CD103 expression in CD11bhighCD11chigh gated cells from (C). (F) CD103 MFI in CD11bhighCD11chigh gated cells from (C) normalized to adult stroma. n = 3 biological replicates. t-test. *p < 0.05.