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. 2021 Mar 30;6:19. doi: 10.1038/s41536-021-00132-4

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Motility of GFP+ macrophages after addition of vehicle, sEVs, and γ-sEVs (Supplementary Movie 1; vehicle, sEVs and γ-sEVs from left to right). b Quantification of morphological changes in macrophages. Data are presented as means ± SD; ten screens were counted in each experiment. CDI, cell deformation index. c Phagocytic ability of DsRed+ macrophages after phagocytosis of pHrodo™ Green Zymosan Bioparticles™ conjugate (Supplementary Movie 2; vehicle, sEVs, and γ-sEVs from left to right). d Frequencies of green fluorescent macrophages observed using time-lapse imaging. Data are presented as means ± SD; ten screens were counted in each experiment 90 min after the addition of sEVs or γ-sEVs. Scale bar = 50 μm. e Change in macrophage polarity after treatment with recombinant human annexin-A1 (ANXA1).