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. 2021 Mar 30;6:19. doi: 10.1038/s41536-021-00132-4

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© The Author(s) 2021

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a The t-distributed stochastic neighbor embedding (t-SNE) graph plotted by combining data for vehicle (PBS), MSC, γ-MSC, sEVs, and γ-sEVs. Red, brown, green, blue, and purple dots show the vehicle (663 cells), MSC (1215 cells), γ-MSC (1505 cells), sEV (1588 cells), and γ-sEV groups (1591 cells), respectively. b Representative cell characters in the clusters shown in a t-SNE plot. c Gene expression in selected macrophage populations. These macrophage populations were divided into four groups (1–4). d–g Frequency of Cd14 + Cd206 + and Cd14 + Cd80 + cells (d), Cx3cr1 + Cd206 + and Cx3cr1 + Cd80 + cells (e), Cd45 + Cd19 + cells in Cd45 + cells (f), and Cd4 + Cd25 + cells in Cd4 + cells (g) in the different groups.