Skip to main content
. 2021 Mar 17;11:633763. doi: 10.3389/fcimb.2021.633763

Figure 10.

Figure 10

Effect of blood on the performance of PCR and RAA. (A) Agarose gel electrophoresis of PCR on blood samples. M, DL2000 DNA marker; 1–10, ASFV positive blood dilutions with double distilled water in a ratio of 1:2, 1:4, 1:8, 1:16, 1:32, 1:64, 1:128, 1:256, 1:512, and 1:1,024. (B) Agarose gel electrophoresis of RAA on blood samples. M, DL2000 DNA marker; N, negative control with double distilled water; 1u–5u, ASFV positive blood dilutions with double distilled water in a ratio of 1:2, 1:4, 1:8, 1:16, 1:32, without boiling and directly used for RAA; 1b–5b, ASFV positive blood dilutions with double distilled water in a ratio of 1:2, 1:4, 1:8, 1:16, 1:32, boiling for 5 min and used for RAA. (C) LFA-RAA for a positive blood samples and a negative one. Blood samples were diluted with PBS in a ratio of 1:3 and boiled for 5 min, follow by RAA reaction. Correct LFA readout indicate blood treatment used in this study was applicable for RAA isothermal amplification and LFA visual readout.