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. 2021 Mar 17;11:633763. doi: 10.3389/fcimb.2021.633763

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Copyright © 2021 Zhang, Li, Guo, Li, Wang, Wang, Xing, Deng and Zhang

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Optimization of RAA-LFA. (A) Optimization of primer combination. 1F1R: the combination of RAA-1F and RAA-1R; 2F1R: the combination of RAA-2F and RAA-1R. 3F1R: the combination of RAA-3F and RAA-1R; 4F1R: the combination of RAA-4F and RAA-1R. 1F2R: the combination of RAA-1F and RAA-2R; 2F2R: the combination of RAA-2F and RAA-2R. 3F1R: the combination of RAA-3F and RAA-2R; 4F2R: the combination of RAA-4F and RAA-2R. (B) Optimization of amplification temperature and time. a1, a2, a3: RAA was performed at room temperature for 5, 10, 15 min. b1, b2, b3: RAA was performed at 37°C for 5, 10, 15 min. c1, c2, c3: RAA was performed at 42°C for 5, 10, 15 min.