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. 2021 Mar 17;11:633763. doi: 10.3389/fcimb.2021.633763

Figure 5.

Figure 5

Sensitivity of OIE-recommended PCR, basic RAA and RAA-LFA for plasmid pUC57-p72. (A) Agarose gel electrophoresis of basic RAA. M: DL2000 DNA marker; 1–9: Decimal dilutions of plasmid pUC57-p72 from 108–100 copies/μL. N, negative control with double distilled water. (B) RAA-LFA visual readout. 1–9: Decimal dilutions of plasmid pUC57-p72 from 108–100 copies/μL. N, negative control with double distilled water. (C) Agarose gel electrophoresis of OIE-recommended PCR. M: DL2000 DNA marker; 1–9: Decimal dilutions of plasmid pUC57-p72 from 108–100 copies/μL. N, negative control with double distilled water. (D) qPCR amplification plot. 1–9: Decimal dilutions of plasmid pUC57-p72 from 108–100 copies/μL. N, negative control with double distilled water.