Table 3.
Significantly regulated genes involved in virulence and biofilm formation in LYSZa7 comparing to P. aeruginosa PAO1 (fold change ≧ 2, FDR adjusted p-value < 0.05); means: normalized mean counts of the sample groups.
| Feature ID | Product | Fold change | Adjusted p-value | PAO1 Means | LYSZa7 Means |
|---|---|---|---|---|---|
| alg44 | alginate biosynthesis protein Alg44 | 11.17 | 3.14E-47 | 26.0 | 277.9 |
| alg8 | alginate biosynthesis protein Alg8 | 7.87 | 8.78E-57 | 53.3 | 389.8 |
| algA | phosphomannose isomerase/guanosine 5’-diphospho-D-mannose pyrophosphorylase | 29.12 | 1.16E-75 | 47.9 | 1352.3 |
| algD | GDP-mannose 6-dehydrogenase AlgD | 19.91 | 7.43E-60 | 56.3 | 1076.1 |
| algE | Alginate production outer membrane protein AlgE precursor | 15.83 | 8.60E-64 | 25.1 | 393.2 |
| algF | alginate o-acetyltransferase AlgF | 19.26 | 5.84E-56 | 14.5 | 322.3 |
| algI | alginate o-acetyltransferase AlgI | 5.19 | 2.60E-23 | 47.9 | 226.6 |
| algJ | alginate o-acetyltransferase AlgJ | 17.14 | 2.28E-46 | 4.8 | 182.5 |
| algK | alginate biosynthetic protein AlgK precursor | 14.33 | 8.76E-48 | 16.2 | 250.3 |
| algL | poly(beta-d-mannuronate) lyase precursor AlgL | 14.69 | 1.29E-58 | 21.6 | 321.1 |
| algX | alginate biosynthesis protein AlgX | 20.54 | 1.60E-59 | 10.8 | 308.2 |
| arr | aminoglycoside response regulator | -318.93 | 1.25E-49 | 229.6 | 2.5 |
| flgL | flagellar hook-associated protein type 3 FlgL | -8.39 | 1.14E-72 | 1,319.7 | 173.3 |
| fliC | flagellin type B | -7.44 | 0.00E+00 | 33,619.2 | 6865.2 |
| fliD | flagellar capping protein FliD | -1,624.06 | 8.80E-207 | 6,412.3 | 5.7 |
| PA2771 | diguanylate cyclase with a self-blocked I-site, Dcsbis | -17.32 | 2.07E-06 | 348.4 | 20.4 |
| PA4625 | cyclic diguanylate-regulated TPS partner A, CdrA | 4.41 | 4.60E-17 | 971.8 | 5208.9 |
| pelD | PelD | 5.55 | 5.54E-35 | 104.8 | 561.9 |
| pelE | PelE | 4.80 | 6.62E-20 | 65.3 | 280.4 |
| pelF | PelF | 5.00 | 8.20E-27 | 88.6 | 414.8 |
| pilA | type 4 fimbrial precursor PilA | -961.48 | 1.61E-221 | 29,476.2 | 40.7 |
| waaL | O-antigen ligase, WaaL | -8.00 | 3.94E-10 | 545.0 | 71.8 |
| wbpA | UDP-N-acetyl-d-glucosamine 6-Dehydrogenase | -1,275.14 | 1.09E-199 | 6,270.7 | 7.2 |
| wbpB | UDP-2-acetamido-2-deoxy-d-glucuronic acid 3-dehydrogenase, WbpB | -1,832.88 | 2.71E-164 | 3,815.0 | 4.8 |
| wbpD | UDP-2-acetamido-3-amino-2,3-dideoxy-d-glucuronic acid N-acetyltransferase, WbpD | -2,685.85 | 6.53E-163 | 1,683.3 | 3.4 |
| wbpE | UDP-2-acetamido-2-dideoxy-d-ribo-hex-3-uluronic acid transaminase, wbpE | -2,156.03 | 6.33E-173 | 4,611.0 | 4.8 |
| wbpG | LPS biosynthesis protein WbpG | -2,523.51 | 3.59E-141 | 4,083.4 | 4.2 |
| wbpH | probable glycosyltransferase WbpH | -1,978.78 | 4.72E-166 | 2,238.8 | 3.7 |
| wbpI | UDP-N-acetylglucosamine 2-epimerase WbpI | -2,058.27 | 6.90E-147 | 3,268.5 | 4.3 |
| wbpJ | probable glycosyl transferase WbpJ | -1,336.39 | 9.86E-162 | 1,523.7 | 4.3 |
| wbpK | probable NAD-dependent epimerase/dehydratase WbpK | -1,800.55 | 2.03E-149 | 1,150.5 | 3.7 |
| wbpL | glycosyltransferase WbpL | -1,912.92 | 3.51E-164 | 1,218.6 | 3.8 |
| wzx | O-antigen translocase | -638.58 | 3.01E-98 | 435.9 | 3.4 |
| wzy | B-band O-antigen polymerase | -6,615.39 | 2.54E-138 | 757.6 | 3.2 |
| wzz | O-antigen chain length regulator | -655.23 | 7.50E-152 | 1,113.7 | 4.5 |
| lasB | elastase LasB | 2.60 | 7.02E-06 | 4,484.2 | 15730.4 |
| rhlA | rhamnosyltransferase chain A | 2.74 | 1.41E-14 | 592.2 | 1887.9 |
| rhlB | rhamnosyltransferase chain B | 3.05 | 5.51E-16 | 294.8 | 940.1 |