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. 2021 Mar 17;12:638454. doi: 10.3389/fpls.2021.638454

FIGURE 1.

FIGURE 1

(A) Schematic illustration of proposed plant HEXO activity on complex LDN-carrying N-glycans. (B) Schematic overview of the expression vectors used in this study. LB, left border; Pnos, nopaline synthase gene promoter; Hyg, hygromycin B phosphotransferase gene; Kan, neomycin phosphotransferase 2 gene; Tnos, nopaline synthase gene terminator; CaMV, cauliflower mosaic virus; d35S, dual cauliflower mosaic virus 35S gene promoter; P35S, cauliflower mosaic virus 35S gene promoter; HEXO, N. benthamiana HEXO ORF; GalNAcT, β1,4-N-acetyl-galactosaminyltransferase from Caenorhabditis elegans; GFP, green fluorescent protein; RFP, red fluorescent protein; XYLT, Arabidopsis thaliana b1,2-xylosyltransferase ORF; S: HEXO RNAi sequence in sense orientation; XTI2, intron 2 from A. thaliana XYLT; AS, HEXO RNAi sequence in antisense orientation; g7T, Agrobacterium gene 7 terminator; and RB, right border.