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. 2021 Mar 17;12:638454. doi: 10.3389/fpls.2021.638454

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Copyright © 2021 Alvisi, van Noort, Dwiani, Geschiere, Sukarta, Varossieau, Nguyen, Strasser, Hokke, Schots and Wilbers.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Expression of Nicotiana benthamiana beta-hexosaminidases (HEXOs). GFP- or RFP-tagged HEXOs or IL-22-GFP (used as apoplast control protein) were transiently expressed in N. benthamiana leaves. As a negative control (–) p19 was used. Leaves were harvested 3 days post infiltration and analyzed by western blot. (A) anti-GFP western blot under reducing conditions of 10 μg total soluble protein of apoplast fluids or intracellular fraction upon expression of IL-22-GFP (22), HEXO2A (2A), HEXO2B (2B), or HEXO3B (3B). (B) anti-RFP western blot under reducing conditions of 10 μg total soluble protein of apoplast fluids or intracellular fraction upon expression of HEXO1A (1A) or HEXO3A (3A).