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. 2021 Mar 17;12:638454. doi: 10.3389/fpls.2021.638454

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Copyright © 2021 Alvisi, van Noort, Dwiani, Geschiere, Sukarta, Varossieau, Nguyen, Strasser, Hokke, Schots and Wilbers.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Cleavage of terminal GlcNAc residues by N. benthamiana HEXOs. Kappa-5 was co-expressed with p19 and different HEXO genes in wild-type plants. After isolation of the apoplast fluid (at 6 dpi) the glycan composition of kappa-5 was analyzed. (A) Griffonia simplicifolia lectin II (GSL-II) binding assay for detection of terminal GlcNAc. Lectin binding is measured as absorbance at 450 nm. (B–D) Kappa-5 was purified from the apoplast fluid and the N-glycan composition was analyzed by MALDI-TOF-MS. MS profiles are given for N-glycans of wild-type kappa-5 (B) or upon co-expression of HEXO1A (C) or HEXO3A (D). m/z refers to mass-to-charge ratio.