Skip to main content
. 2021 Mar 17;12:638454. doi: 10.3389/fpls.2021.638454

FIGURE 6.

FIGURE 6

Transient silencing of HEXO2 and HEXO3. Kappa-5 was co-expressed with CeGalNAcT and constructs targeting HEXO2 (Δ2), HEXO3 (Δ3), or both (Δ2/3). The samples are grouped in three blocks depending on whether a HEXO variant (2B or 3A) was co-expressed. Control samples where a particular construct is not co-infiltrated is indicated as well (–). After isolation of the apoplast fluid (at 5 dpi) the presence of the LDN motif on kappa-5 N-glycans was analyzed. (A) Soybean agglutinin (SBA) binding assay for detection of terminal GalNAc. Lectin binding is measured as absorbance at 450 nm. (B,C) Kappa-5 was purified from the apoplast fluid and the N-glycan composition was analyzed by MALDI-TOF-MS. MS profiles are given for glucosaminidase treated N-glycans of kappa-5 upon co-expression of GalNAcT only (B) or combined with the hairpin construct targeting HEXO2 and HEXO3 (Δ2/Δ3; C). m/z refers to mass-to-charge ratio.