Table 2.

In vitro micronucleus results after Lacprodan® BLG exposure.

Exposure	Concentration (μg/mL)	CBPI slide 1 / slide 2	Relative cell growth (%)a	Micronucleated cells (%) culture 1 /culture 2	Average number of micronucleated cells (%)	Historical negative control limits
Experiment I (– S9)						0.29 % - 1.16 %
	0	1.51 / 1.40	100	0.20 / 0.30	0.25
	1000	1.36 / 1.38	81	0.70 / 0.30	0.50
	1500	1.48 / 1.58	116	1.10 / 0.40	0.75*
	2000	1.44 / 1.50	102	0.40 / 0.40	0.40
Positive controls
MMS	65	1.38 / 1.38	83	4.90 / 1.75	3.33*
Colchicine	0.8	1.19 / 1.15	37	3.60 / 5.80	4.70*
Experiment I (+S9)						0.28 % - 1.26 %
	0	1.46 / 1.47	100	0.90 / 0.40	0.65
	500	1.44 / 1.48	100	0.50 / 0.80	0.65
	1000	1.45 / 1.44	96	0.70 / 0.70	0.70
	1500	1.44 / 1.42	93	1.00 / 0.40	0.70
	2000	1.40 / 1.59	108	0.60 / 1.00	0.80
Positive control
CPA	15	1.28 / 1.28	60	2.30 / 3.70	3.00*
Experiment II (– S9)						0.29 % - 1.16 %
	0	1.60 / 1.71	100	0.90 / 0.70	0.80
	1000	1.60 / 1.62	93	1.30 / 0.30	0.80
	1500	1.65 / 1.60	96	1.00 / 0.90	0.95
	2000	1.72 / 1.58	99	0.60 / 1.20	0.90
Positive controls
MMS	50	1.44 / 1.61	80	3.70 / 5.10	4.40*
Colchicine	0.04	1.23 / 1.22	34	4.60 / 6.49	5.55*

CBPI = ((c₁ x 1) + (c₂ x 2) + (c_x x 3)) / n, where c_1, c₂ and c_x are mononucleate, binucleate and multinucleate cells, respectively, and n = total number of cells.

Relative cell growth = 100 x ((CBPI_{test conc.} – 1) / (CBPI_control – 1)).

^*P < 0.05 as compared with control (Chi-square test). MMS = methylmethanesulfonate; CPA = cyclophosphamide.

^aRelative to negative control.