Figure 7.

Genetic depletion of Isx gene restores retinoid biosynthesis in Lrat^−/− mice. Lrat^−/− and Lrat^−/−/Isx^−/− (double knockout) mice (n = 4–5 per genotype) were fed with β-carotene (BC) diet for 4 weeks. A–C: Quantification of the concentration of all-trans-retinol (ROL), retinyl esters (REs), and β-carotene (BC) in jejunum of the mice. D and E: ROL concentration of the liver and the gonadal adipose depots. F–H: Quantification of the concentration of ROL, RE, and BC of mesenteric lymph nodes. The data represent means and ± SD. ∗∗∗P < 0.0001 using unpaired two-tailed t-test comparing each genotype. nd, not detectable.