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. 2021 Mar 17;12:633333. doi: 10.3389/fpls.2021.633333

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Copyright © 2021 Jia, Wang, Wang, Ye, Liu, Jiang, Sun, Yan, Wang, Wang and Fan.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Y1H assays of DcTTG1 and anthocyanin biosynthesis-related gene promoters. The DcTTG1 sequence and promoter fragments of nine anthocyanin biosynthetic genes were cloned into pGADT7 and pHIS2 vectors, respectively. The plasmids were transformed into Y187 yeast cells (Clontech Laboratories, Mountain View, CA, United States) that were grown on SD (–Leu/–Trp/–His) medium at 28°C for 3 days. The optimal 3-AT concentration (in mM) to suppress background histidine leakiness after addition to the (–Leu/–Trp/–His) medium is indicated in the lower-right corner of each picture.