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. 2021 Mar 17;11:634881. doi: 10.3389/fonc.2021.634881

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Copyright © 2021 Jin, Zhang, Zou, Li, Chen, Peng, Deng, Wen, Shen and Zhan.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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SHR-A1403 inhibits cholesterol biosynthesis in pancreatic cancer cells in vitro. (A) Heatmap shows the upregulated and downregulated genes in the cholesterol biosynthesis pathway in cells treated with SHR-A1403 compared to control-treated cells. (B–D) qRT-PCR analysis of key enzymes in the biosynthesis of cholesterol in PDAC cells treated with SHR-A1403. We examined enzymes involved upstream and downstream of biosynthesis, as well as those regulating intermediate metabolites. (E) Western blotting verified the expression of some key enzymes upstream and downstream of cholesterol biosynthesis in PDAC cells treated with SHR-A1403. (F) Fluorescence semi-quantitative detection of cholesterol content in Aspc-1 cells treated with different concentrations of SHR-A1403. (G,H) A cholesterol quantification kit was used to quantify the total cholesterol and free cholesterol content in Aspc-1 cells after treatment with different concentrations of SHR-A1403. *P < 0.05, **P < 0.01, ***P < 0.001. qRT-PCR, quantitative reverse transcription polymerase chain reaction; PDAC, pancreatic ductal adenocarcinoma.