FIGURE 4.

Sex-differences in T cell and macrophage subtypes in PVATs peak at 17 weeks on diet, with the onset of hypertension. Subtypes of CD4 +, CD8 + T cells and CD68 + macrophages in MRPVAT and APVAT of both sexes at 17 weeks on diet (onset of hypertension with HFD) were quantified by flow cytometry. Subtypes of CD4 + T cells (A,D), CD8 + T cells: cells (B,E) and CD68 + macrophages (C,F) were measured. Bars represent means ± SEM for the absolute cell counts of each immune cell type, normalized to tissue weight (in milligram). Number of animals is indicated by N. A P < 0.05 by 2-way ANOVA with Tukey’s test for multiple comparison, was considered statistically significant. * and † inside/outside the stacks in the graph represent a significant difference in the specific stack of immune cells/sum total of immune cells between the sexes within each diet and between the diets within each sex respectively.