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. 2021 Mar 17;12:616055. doi: 10.3389/fphys.2021.616055

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Copyright © 2021 Kumar, Yang, Contreras, Garver, Bhattacharya, Fink, Rockwell and Watts.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Sex-differences in immune cells diminish with the progression of hypertension after 24 weeks on diet. Subtypes of CD4 +, CD8 + T cells and CD68 + macrophages in MRPVAT and APVAT of both sexes at 24 weeks on diet (hypertensive with HFD) were quantified by flow cytometry. Subtypes of CD4 + T cells (A,D), CD8 + T cells: cells (B,E), and CD68 + macrophages (C,F) were measured. Bars represent means ± SEM for the absolute cell counts of each immune cell type, normalized to tissue weight (in milligram). Number of animals is indicated by N. A P < 0.05 by two-way ANOVA with Tukey’s test for multiple comparison, was considered statistically significant. * and † inside/outside the stacks in the graph represent a significant difference in the specific stack of immune cells/sum total of immune cells between the sexes within each diet and between the diets within each sex respectively.