FIGURE 6.

Magnitude of expression of markers of M2-like macrophages and T regulatory cells over the time course of hypertension development and progression. Mean fluorescence intensities (MFI) of CD163 on CD163 + M2-like macrophages (A,D), Foxp3 on CD4 + (B,E) and CD8 + (C,F) T regulatory cells in MRPVAT and APVAT of both sexes over the time course on diet were quantified by flow cytometry. Points represent means ± SEM of the magnitude of expression (measured by MFI) of the specified marker. Number of animals is indicated by N. A P < 0.05 by two-way ANOVA with Tukey’s test for multiple comparison, was considered statistically significant. Circles in the graph represent a significant difference in MFI between the sexes within a diet at a given time point.