Figure 1.

Scheme of nLD protein purification and identification. A schematic flowchart of nLD protein extraction, concentration, and identification is illustrated as explained in the Materials and Method section. nLD were isolated from rat-liver nuclei and corresponded to the sucrose-gradient upper band (α band). Three different subsets of nLD proteins were obtained: Sample A (A), the total proteins in the nLD fraction, comprising those from the nLD as well as the floating buoyant nuclear proteins; Sample B (B), the nLD-associated proteins; and Sample C (C), the nLD salt-resistant proteins. A total of 35 different proteins were identified among the three nLD samples; with 17, 16, and 14 total proteins comprising samples A, B, and C, respectively, as detailed in Supplementary Tables S1–S3. A total of 12, 8, and 6 unique proteins were identified in A (Only A), B (Only B), and C (Only C) samples, respectively; 1, 1, and 4 proteins were present in both samples A and B, A and C, and B and C, respectively; while 3 proteins were identified in all three nLD samples (A, B, and C) as detailed in Supplementary Table S4.