Figure 3.

Structural representation of LDH tetrameric interface shows that cluster B₁continuous epitope is a long α-helix. Representation of clusters A₁ and B₁ (blue, ribbon) interacting with clusters A₂ and B₂ (orange, surface). The surface corresponds to the molecular surface of LDH-H clusters A₂ and B₂ colored by lipophilicity (gray blue: lipophilic; pink: hydrophilic). This representation was generated from the LDH-1 crystallographic structure and further minimized using Molecular Operating Environment software (Protein Data Bank ID: 1I0Z). LDH, lactate dehydrogenase; LDH-1, lactate dehydrogenase heart isozyme homotetramer; LDH-H, lactate dehydrogenase heart isozyme.