Table 1.
Motility, velocity parameters, membrane integrity, and membrane functionality of rooster sperm treated with 0, 1, 2, and 4 mmo concentrations of methyl-beta-cyclodextrin (MBCD) before transfection.
| Characteristics1 | Control | 1 mmol | 2 mmol | 4 Mm | SEM |
|---|---|---|---|---|---|
| TM (%) | 91.6b | 97.3a | 96.3a | 89.2b | 2.23 |
| PM (%) | 60.7b | 67.3a | 66.1a | 59.8b | 1.12 |
| VCL (μm/s) | 107.9 | 108 | 105.4 | 106 | 2.45 |
| VSL (μm/s) | 55.6b | 64.9a | 68.1a | 55b | 3.1 |
| STR (%) | 77.5 | 74.8 | 72.9 | 73 | 2.95 |
| LIN (%) | 60.7 | 62.9 | 65.8 | 61.3 | 1.7 |
| MI (%) | 93.2b | 98.4a | 96.9a | 88.2b | 3.03 |
| Membrane functionality (%) | 92.4 | 93.1 | 90.5 | 91.7 | 3.8 |
a-cMeans within rows with different superscripts are significantly different (P < 0.05).
1
TM: Total motility; PM: Progressive motility; VCL: Curvilinear velocity; VSL: Straight linear velocity; STR: Sperm track straightness; LIN: Linearity.