Figure 2.

GC-induced gene expression in MKO mice. Muscle specific Pik3r1 knockout (MKO) mice were generated. A, the expression of Pik3r1 in gastrocnemius (GA) muscle, tibialis anterior (TA) muscle, and soleus muscle of WT and MKO was examined by immunoblots and normalized to internal control Gapdh. Representative immunoblots are shown (n = 3). B, the expression of Sesn1 and Fkbp5 was evaluated in WT and MKO mouse gastrocnemius muscle treated with PBS or DEX (10 mg/kg bodyweight) in drinking water for 1 week. Error bars represent SD, n = 5 to 6 and ∗p ≤ 0.05. DEX, dexamethasone.