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. 2021 Mar 16;12:629242. doi: 10.3389/fendo.2021.629242

Table 1.

SNP genotyping of the lyp region in the DRLyp/Lyp rats.

Position Dist. Mutation Forward Primer Seq. Reverse Primer Seq. Rep 1 Seq. (DR+/+) Rep 2 Seq. (DRLyp/Lyp)
77 630 116 -753 877 GCAGGAGAGGCTGCTATGTC TCCACAGCATGCCAAGCT CCACAGGAGAGTTGTG CACAGGAGACTTGTG
78 170 204 -213 789 CCTCCCTTGGTATGTTGTGTATGAT TGCCAGGGATAGCAGAGTGA TCCCTTCTGTGTCCAGTAC CCTTCTGCGTCCAGTAC
78 377 812 -6 181 GGTAAATACTTCACCCTGCTTCCA GCTGGTTACCCCTTCTTTACTATTTGA CATTATGGAGTTTCTAGCCTAT ATGGAGTTTCTCGCCTAT
78 383 993 0 ACATGGGAGGGAAGGAGCTT CATGTCTTGGTTCTGGATCTTTGAC ACGCCCCCCATCTT CACGCCCCCATCTT
78 386 587 2 594 GATACGGGAAAGTACAGGGTAGACT TCAGTTTTTGAAAATGACATCTAAAGTTTCTGT ATTCTCTCCGACCCCAGTT ATTCTCTCCGAACCCAGTT
78 576 508 192 515 CCCTACTACCTGCCCTTTAGAAG GACCTGCTTATTTCTGGAGTGGATT TCTTCAGATGTACTTTTAG CTTCAGATGTCCTTTTAG
79 071 296 687 303 CCATACCATCTCTATCATCCTGTTTCC GGGCTACAAAACAAAGCAGAAAGTT AATCTGGGAAAGGCA TGAATCTGAGAAAGGCA

Distance mutation is the number of base pairs from the Gimap5 frame shift mutation (0). The forward and reverse primer sequences used to identify the SNP between DR.+/+ (wild type) and DRLyp/Lyp are shown.