Table 1.
Position | Dist. Mutation | Forward Primer Seq. | Reverse Primer Seq. | Rep 1 Seq. (DR+/+) | Rep 2 Seq. (DRLyp/Lyp) |
---|---|---|---|---|---|
77 630 116 | -753 877 | GCAGGAGAGGCTGCTATGTC | TCCACAGCATGCCAAGCT | CCACAGGAGAGTTGTG | CACAGGAGACTTGTG |
78 170 204 | -213 789 | CCTCCCTTGGTATGTTGTGTATGAT | TGCCAGGGATAGCAGAGTGA | TCCCTTCTGTGTCCAGTAC | CCTTCTGCGTCCAGTAC |
78 377 812 | -6 181 | GGTAAATACTTCACCCTGCTTCCA | GCTGGTTACCCCTTCTTTACTATTTGA | CATTATGGAGTTTCTAGCCTAT | ATGGAGTTTCTCGCCTAT |
78 383 993 | 0 | ACATGGGAGGGAAGGAGCTT | CATGTCTTGGTTCTGGATCTTTGAC | ACGCCCCCCATCTT | CACGCCCCCATCTT |
78 386 587 | 2 594 | GATACGGGAAAGTACAGGGTAGACT | TCAGTTTTTGAAAATGACATCTAAAGTTTCTGT | ATTCTCTCCGACCCCAGTT | ATTCTCTCCGAACCCAGTT |
78 576 508 | 192 515 | CCCTACTACCTGCCCTTTAGAAG | GACCTGCTTATTTCTGGAGTGGATT | TCTTCAGATGTACTTTTAG | CTTCAGATGTCCTTTTAG |
79 071 296 | 687 303 | CCATACCATCTCTATCATCCTGTTTCC | GGGCTACAAAACAAAGCAGAAAGTT | AATCTGGGAAAGGCA | TGAATCTGAGAAAGGCA |
Distance mutation is the number of base pairs from the Gimap5 frame shift mutation (0). The forward and reverse primer sequences used to identify the SNP between DR.+/+ (wild type) and DRLyp/Lyp are shown.