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. 2021 Mar 17;13:653379. doi: 10.3389/fnagi.2021.653379

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Copyright © 2021 Jia, Cheng, Li, Chen, Yang, Liu, Li and Le.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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α-SYN regulated the expression of Sp1. Western blot was used to detect the protein level of Sp1 in control cells (Vector), α-SYN^WT and α-SYN^A53T stably overexpressed cells (A,B). The intracellular protein level of Sp1 was confirmed using immunofluorescent staining, α-SYN (red), Sp1 (green), and nucleus (blue). Scale bare: 50 μm (C). Western blot detected the cytoplasm and nucleus protein level of Sp1 in control cells (Vector), α-SYN^WT and α-SYN^A53T stably overexpressed cells (D–F). Data were expressed as mean ± SEM. *p < 0.05 vs. Vector transfected cells, n = 3.