. 2021 Mar 17;12:636048. doi: 10.3389/fphar.2021.636048

TABLE 4.

Comparison of pharmacodynamic variables measured as calcineurin (CN) activity and their corresponding correlations after morning tacrolimus dose (0–12 h) and after night Tac dose (12–24 h) of twice-daily tacrolimus formulation.

Variables	Morning dose (0–12 h)	Night dose (12–24 h)	p
I_trough (pmol RII/min·mg prot)	296.0 [277.1–316.3]	285.8 [266.1–307.0]	0.040 ^a
I_nadir (pmol RII/min·mg prot)	220.1 [204.3–237.2]	238.7 [221.7–257.0]	0.002 ^a
T_nadir (h)	2.16 [1.25–3.49]	2.76 [1.47–4.26]	0.656 ^b
I_trough/I_nadir	1.29 [1.25–1.34]	1.20 [1.15–1.24]	0.002 ^a
AUE I_min (pmol RII·h/min·mg prot)	127.7 [112.3–145.2]	120.1 [99.1–145.6]	0.572 ^a
AUE I_nadir (pmol RII·h/min·mg prot)	262.4 [216.2–318.4]	245.9 [190.6–317.3]	0.449 ^a
Correlations ^c	Morning dose (0–12 h)		Night dose (12–24 h)
	R	p	R	P
I₀ vs AUE_0–12 h I_min	−0.034	0.881	—	—
I₁₂ vs AUE_0–12 h I_min	0.115	0.609	—	—
I₁₂ vs AUE_12–24 h I_min	—	—	−0.001	0.968
I₂₄ vs AUE_12–24 h I_min	—	—	−0.255	0.252
I₀ vs AUE_0–12 h I_nadir	−0.134	0.552	-	-
I₁₂ vs AUE_0–12 h I_nadir	0.125	0.579	-	-
I₁₂ vs AUE_12–24 h I_nadir	—	—	0.158	0.491
I₂₄ vs AUE_12–24 h I_nadir	—	—	0.252	0.257
I₀ vs I_{nadir 0–12 h}	0.899	<0.001
I₁₂ vs I_{nadir 12–24 h}			0.818	<0.001

^{^a}

Paired t-test.

^{^b}

Wilcoxon test.

^{^c}

Pearson’s correlation test.

Data is represented as geometric mean [95% CI] unless T_nadir that is expressed as median [interquartile range]. I_trough, trough CN activity at time 12 h for morning dose and at time 24 h for night dose; I_nadir, maximum inhibition of CN activity; T_nadir, time to reach I_nadir; AUE I_min, area under the effect-time curve of CN inhibition from each 12 h dose interval using I_min as baseline; I_min, minimum CN inhibition observed along 24 h interval; AUE I_nadir, area under the effect-time curve of CN inhibition from each 12 h dose interval using the I_nadir observed along 24 h interval as baseline; I₀, morning pre-dose CN activity at time 0 h; C₁₂, night pre-dose CN activity at time 12 h; C₂₄, CN activity concentration at time 24 h; AUE_0–12 h, area under the effect-time curve after the morning dose from 0 to 12 h; AUE_12–24 h, area under the effect-time after the night dose from 12 to 24 h.