Refeeding and fasting after ethanol withdrawal induced distinctive changes in LD membrane proteome content. A: Principal component analysis (PCA) compares control (blue), ethanol (green), 7-day (red), control-fast (yellow), and EtOH-fast (purple) groups. Volcano plot show magnitude of fold changes of proteins significantly (P < 0.05) altered in LDs from (B) 7 days refed (C) control fast, and (D) ethanol fast. E: The heat map show changes induced by ethanol and 7-day refeeding and fasting of ethanol-fed rats after ethanol withdrawal, to selected bona fide and LD-associated lipid-metabolizing proteins. LD, lipid droplet.