Fig. 8.

Refeeding and fasting after ethanol withdrawal promoted mitochondria and lysosomal function by restoring nuclear levels of transcription factor EB (TFEB). A: Immunostaining of liver sections with the antibody to lysosomal marker protein LAMP1 (red) and lipid droplet marker protein Plin-2 (green), from rats treated, as indicated. Fluorescence intensity quantification by ImageJ of (B) total lysosomal staining, (C) lysosomal staining around lipid droplets, and (D) specific activity of LAL in lysosomes isolated from livers of rats treated as indicated on the graph. E: TFEB in nuclear fractions. Data are the means ± SE of 6–8 animals/group. Bars with different letters are significantly different. Bars with the same letter are not significantly different, P ≤ 0.05. LAL, lysosomal acid lipase; LAMP, lysosome-associated membrane protein; PLIN, perilipin.