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. 2021 Feb 20;296:100447. doi: 10.1016/j.jbc.2021.100447

Figure 2.

Figure 2

Circular dichroism (CD) thermal melts of (A) Adnectin-anti-VEGFR2 (Tmof 50 °C ± 0.4 °C) and (B) FN3Con-anti-VEGFR2 (Tmof 89 °C ± 0.2 °C), a reverse melt (blue) indicates reversible refolding in FN3Con-anti-VEGFR2.C, the Adnectin-anti-VEGFR2 sample aggregates completely over 1 month at 36 °C. D, more than 1/3 of FN3Con-anti-VEGFR2 sample is still present as a monomer after 2 years of 36 °C storage, with the remaining 2/3 present as higher-order oligomers. E, the graft also shows limited aggregation during 4 °C storage. F, ELISA of the 36 °C 24-month sample shows a matching threefold loss in affinity (KD of 49–157 nM).