Figure 4.

A, change in protein T_mas loops of the Adnectin scaffold were evolved for stronger binding to VEGFR2 (40, 41), transfer of binding loops to FN3Con retained similar affinity while heavily improving thermostability. Data in Table S1. B, this improvement in thermal stability produced a related increase in LTS under elevated temperatures. The Adnectin aggregated with no stable oligomers, so later monomer percentages were compared against the day 1 values (green). As FN3Con remained at ∼1 mg/ml over the course of each trial, monomer percentages were calculated from the total peak area within each timepoint (red, gray).