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. 2021 Feb 20;296:100447. doi: 10.1016/j.jbc.2021.100447

Table 1.

Methodology and results for VEGFR2 binding experiments in Figure 1

Protein Surface plasmon resonance (SPR) KD ELISA KD
Adnectin-anti-VEGFR2 0.31 nMa 16.87 nM ± 4.26
FN3Con-anti-VEGFR2 0.72 nM ±0.21 48.79 nM ± 6.78
Analysis
 Fold Difference 2.32 2.89
 FN3Con-anti-VEGFR2 Model Fit R2 = 0.95 R2 = 0.98
 Immobilized protein VEGFR2 VEGFR2
 Measure Change in refractive index Concentration of Biotinylated protein
a

SPR KD from (41), ELISA values are KD ± SE.