Table 1.
Methodology and results for VEGFR2 binding experiments in Figure 1
| Protein | Surface plasmon resonance (SPR) KD | ELISA KD |
|---|---|---|
| Adnectin-anti-VEGFR2 | 0.31 nMa | 16.87 nM ± 4.26 |
| FN3Con-anti-VEGFR2 | 0.72 nM ±0.21 | 48.79 nM ± 6.78 |
| Analysis | ||
| Fold Difference | 2.32 | 2.89 |
| FN3Con-anti-VEGFR2 Model Fit | R2 = 0.95 | R2 = 0.98 |
| Immobilized protein | VEGFR2 | VEGFR2 |
| Measure | Change in refractive index | Concentration of Biotinylated protein |
SPR KD from (41), ELISA values are KD ± SE.