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. 2021 Mar 29;11(2):20458940211000234. doi: 10.1177/20458940211000234

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© The Author(s) 2021

Creative Commons Non Commercial CC BY-NC: This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Fig. 3. — CircHIPK3 regulated the function of PDGF-treated hPAECs. (a) The expression level of CircHIPK3 transfected with siRNA-1, siRNA-2, and siRNA-3 was verified by qRT-PCR. (b) Effect of PDGF treatment on circHIPK3 expression. CircHIPK3 siRNA reduced the expression of circHIPK3 in PDGF-treated hPAECs. (c) CCK-8 assay was performed to detect the proliferation of hPAECs under and circHIPK3 knockdown. (d) The hPAECs migration was measured using a Transwell assay, scale bar = 25 μm. (e) The effect of circHIPK3 on tube formation assay in hPAECs treated with PDGF, scale bar = 25 μm. (f and g) The qRT-PCR and western blot were used to detect bFGF and VEGF expression at mRNA and protein level. Data were expressed as mean ± SD in three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001. PGDF: platelet-derived growth factor; VEGF: vascular endothelial growth factor; bFGF: basic fibroblast growth factor; hPAEC: human pulmonary artery endothelial cell; GADPH: glycerldehyde-3-phosphate dehydrogenase.