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. 2021 Mar 29;11(2):20458940211000234. doi: 10.1177/20458940211000234

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© The Author(s) 2021

Creative Commons Non Commercial CC BY-NC: This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage).

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Fig. 6. — CircHIPK3 promoted the function of hPAECs through regulating the miR-328-3p/STAT3 axis. (a) qRT-PCR analysis for circHIPK3 and miR-328-3p in hPAECs from each group. (b) qRT-PCR detected the expression of STAT3 in hPAECs from each group at the mRNA level. (c) Western blot analysis for STAT3 protein in hPAECs from each group. (d) CCK-8 assay was performed to detect cell proliferation in different groups. (e) Transwell assay was used to measure cell migration in different groups, scale bar = 25 μm. (f) Representative images and graph of the numbers of tubules formed in each group, scale bar = 25 μm. Data were expressed as mean ± SD in three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001. STAT3: STAT3 overexpression vector pcDNA-STAT3; NC: negative control; OD: optical density.