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. 2021 Mar 31;19:94. doi: 10.1186/s12951-021-00840-5

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 4 — LbL coating maintained HFSC properties. a, b Flow cytometry was conducted on day 7 after culture. The results showed that the proportion of CD200⁺a6⁺ HFSCs at P0 or P2 in the coated group was higher than the uncoated group (mean ± SD; n = 3; **p < 0.01, Student’s t-test). c, d Immunofluorescence staining was carried out on day 7 after culture. The proportion of CD200⁺a6⁺ HFSCs at P0 or P2 in the coated group was higher (mean ± SD; n = 4; **p < 0.01, Student’s t-test). DAPI (blue); CD200 (red); a6 (green); scale bars: 20 µm. e The long-term cell culture under LbL coating results are from flow cytometry analysis of cells in each passage (mean ± SD; n = 3)