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. 2021 Mar 31;19:94. doi: 10.1186/s12951-021-00840-5

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 5 — FGF-7 and VEGF-A stabilize the CD200⁺α6⁺ HFSC population. a, b Flow cytometry conducted on the seventh day after culture at P2. The ratio of CD200⁺a6⁺ HFSCs in the LbL-HFSCs + FV group was higher than the other groups (mean ± SD; n = 3; **p < 0.01, one-way ANOVA). c, d Immunofluorescence staining performed on the seventh day after culture at P2. Red-stained CD200⁺ HFSCs in the LbL-HFSCs + FV group was higher than the other groups (mean ± SD; n = 4; **p < 0.01, one-way ANOVA). DAPI (blue); CD200 (red); scale bars: 20 µm. e Absolute numbers of CD200⁺a6⁺ HFSCs at P2 cultured under different conditions, indicated as fold-change relative to the number of cells inoculated at P0 (mean ± SD; n = 4; **p < 0.01, one-way ANOVA). f The results of the long-term cell culture performed under different conditions are from flow cytometry analysis of cells in each passage. Only the LbL-HFSCs + FV culture maintained a stable CD200⁺a6⁺ HFSC population for an extensive period after P2 (mean ± SD; n = 3)