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. 2021 Mar 31;20:78. doi: 10.1186/s12934-021-01568-5

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 1 — Operational taxonomic units (OTUs) and sample feasibility analysis. Venn diagrams show unique and shared bacterial OTUs in jejunum (a) the jejunum in control group; (c) the jejunum in milk replacer-treated group) and cecum (b) the cecum in control group, (d) the cecum in milk replacer-treated group). Venn diagrams for core OTUs distribution in all the jejunum (e) and cecum (f). Rank-Abundance (g) and rarefaction curves (h, i) were used for the assessment of sequencing depth. Each color-curve represents a sample. B15.2, B25.2, B45.2 and B75.2 represent the jejunum in the control group on the days 15, 25, 45 and 75, respectively. B15.4, B25.4, B45.4 and B75.4 represent the cecum in the control group on the days 15, 25, 45 and 75, respectively. R15.2, R25.2, R45.2 and R75.2 indicate the jejunum in the milk replacer-treated group on the days 15, 25, 45 and 75, respectively. R15.4, R25.4, R45.4 and R75.4 indicate the cecum in the milk replacer-treated group on the days 15, 25, 45 and 75, respectively