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. 2021 Mar 31;12:221. doi: 10.1186/s13287-021-02290-0

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© The Author(s) 2021

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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Fig. 2 — The effect of ADSC-Exo on HSFs. a, b Cell cycle analysis of HSFs exposed to ADSC-Exo or PBS. c Representative images of immunofluorescence staining of Ki67 in HSF exposure to ADSC-Exo or PBS, scale bar = 50 μm. d, e The effect of ADSC-Exo on HSF migration evaluated by the scratch wound assays and crystal violet staining, graphs showed the comparison of relative wound areas between ADSC-Exo and control group at 0 h and 24 h, original magnification (× 4 or × 10). f qRT-PCR analysis of the profibrotic factors in HSFs treated with ADSC-Exo or PBS, graph represented the expression of Col1, Col3, and α-SMA relative to that of GAPDH. g, h Immunoblot analysis of Col1, Col3, and α-SMA in HSFs stimulated with ADSC-Exo or PBS, the histogram demonstrated the relative band density to β-actin. i Representative images of α-SMA immunofluorescence staining in HSFs stimulated with ADSC-Exo or PBS, scale bar = 50 μm. The data was shown as mean ± SEM (*p < 0.05, **p < 0.01, ns: no differences)